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CytoFa

 

 

From a single experiment to complex fully automated microscope imaging and liquid handling processes: CytoFa is the ideal system for your application. Examine your samples under the microscope, take microscope images, pipette liquids, or do all with an automatic sequence protocol. In particular, antibody staining of tissue sections can be automatically performed and analyzed with CytoFa. The motorized upright fluorescence microscope of Zeiss Company ensures excellent optical properties for image acquisition. With the robot-controlled electronic pipette small volumes can be directly applied to the samples on the microscope stage. This is not only efficient, but also saves costs in the use of expensive reagents.

 

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Applications

 

CytoFa is the perfect system for Toponome research. The patented method allows the visualization of up to 100 molecular parameters of a cell or a tissue section. Learn about Toponome and the Human Toponome project (HUTO)

 

 

The motorized upright fluorescence microscope Axio Imager of the company Zeiss can be equipped according to the application and the budget. The current microscope configuration is read automatically and the single components are displayed and can be controlled by the CytoFa software.

 

 

Basic configuration

 

Illumination

 

  • VIS-LED lamp for transmitted light
  • Zeiss Colibri.2 LED system for epi-fluorescence (free choice of up to 4 different LED modules and filter sets)


Contrast methods

 

  • Bright field
  • Phase contrast

 

Objectives

 

  • 25x/0,8 Immersion objective, Ph2, M27
  • 63x/1,0 Immersion objective, Ph3, M27

 

Motorized components

 

  • Mechanical stage and Z-drive
  • Illumination (transmitted/reflected light, on/off/brightness of LED lamp and LED modules)
  • Objective nosepiece
  • Condenser
  • Reflector turret
  • Sideport/Beam splitter

The high-resolution monochrome CCD camera is mounted on the sideport of the microscope. Sensor cooling ensures low noise and high quality images. With the CytoFa software also single images can be taken.

 

  • SciCam 4022, 16 Bit, Electronic Shutter
  • USB2.0, max. Download speed: 12 MHz
  • Array size: 2048 x 2048
  • Pixel size: 7.4 µm
  • Sensor Diagonal: 21.4 mm

When you have found an interesting detail of your sample with the microscope then bring it into the focus and simply press a button in the CytoFa software: This field of view is saved and will be used for each image acquisition in the automatic sequence. Up to four visual fields can be defined by the user. Additional key features are:

 

  • Auto-focus function adjustable to the type of sample (cells/tissue section).
  • Option to take images of z planes (z-stack).
  • Image acquisition of the same visual field with different microscope settings (for example, phase contrast image, different fluorescence images and exposure times)
  • Optimization tools (for example, reading the current microscope setting, manual control of the camera to test different exposure times).

The handling of the liquid is carried out with an electronic pipette connected to a robot system. Volume uptake and release are user-specific and dependent on the used pipette tip. The solutions can be stored in three 96 deep well plates on a platform or in a 96 well plate at a defined temperature on a thermostat. The key features are:

 

  • Easy exchange of the pipette tip via menu navigation.
  • Manual operation of the electronic pipette via the CytoFa software.
  • Integrated robot safety concept to avoid collisions.

With the easy-to-use CytoFa-Software all key functions can be controlled:

 

  • Control of single components (for example single image acquisition).
  • Execution of partial sequences (for example multiple liquid exchange).
  • User-defined running log for automatic sequences (for example pipetting step followed by image acquisition, incubation, and waiting time).

The running log for automatic sequences can be saved and enables an easy setup and optimization of identical or similar experiments due to fast and easy modification of single parameters. In addition, the runtime log is displayed for final check and can be copied to other documents. Parallel, a text file of the runtime log is automatically created with the start that can be imported into other applications.